质粒名称: | pQFlag-USP7 WT puroR |
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出品公司: | 爱迪基因 |
目录编号: | 46751 |
存储实验室: | Goedele Maertens, Gordon Peters |
载体骨架基本信息 |
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载体名称: | pQCXIP |
载体出品公司: | Clontech |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | 10491 bp |
载体修饰: | NLS(插入物上的C末端)pQFlag-puro通过连接在NotI-和BamHI消化的pQCXIP中进行工程化,产生以下MC:NotI Kozak MfeI Flag AgeI XhoI BamHI |
载体类型: | 哺乳动物表达,逆转录病毒 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | -- |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | USP7 |
物种来源: | H. sapiens (human) |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | CMV |
标签/融合蛋白: | Flag(主干上的N终端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | AgeI(未破坏) |
3' 克隆位点: | BamHI (未破坏) |
5' 测序引物 | CMV Forward |
3' 测序引物 | pCDH-Rev |
文献引用方法 |
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材料和方法部分: | pQFlag-USP7 WT puroR was a gift from Goedele Maertens & Gordon Peters (Addgene plasmid # 46751 ; http://n2t.net/addgene:46751 ; RRID:Addgene_46751) |
参考文献部分: | Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G. EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub 2010 Jul 2. 10.1038/emboj.2010.129 PubMed 20601937 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pQFlag-USP7 WT puroR 10495 bp ds-DNA circular SYN 11-JUN-2019 DEFINITION Retroviral vector that expresses wild type Flag-tagged human USP7. ACCESSION . VERSION . KEYWORDS pQFlag-USP7 WT puroR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10495) AUTHORS Maertens GN, El Messaoudi-Aubert S, Elderkin S, Hiom K, Peters G TITLE Ubiquitin-specific proteases 7 and 11 modulate Polycomb regulation of the INK4a tumour suppressor. JOURNAL EMBO J. 2010 Aug 4;29(15):2553-65. doi: 10.1038/emboj.2010.129. Epub 2010 Jul 2. PUBMED 20601937 REFERENCE 2 (bases 1 to 10495) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 11, 2019 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10495 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 309..512 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" LTR 513..688 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 751..950 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1151..1567 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1497..1519 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1535..1551 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" enhancer 1604..1907 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1908..2111 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2108..2132 /label=LNCX /note="Human CMV promoter, forward primer" CDS 2268..2291 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" misc_feature 5622..6195 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5789..5806) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6016..6035 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6228..6827 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6228..6247) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6724..6744 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 7201..7626 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from Moloney murine leukemia virus" primer_bind complement(7729..7751) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7887..7907) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7892..8221 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 8072..8207 /label=SV40 ori /note="SV40 origin of replication" primer_bind 8134..8153 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 8243..8262 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(8343..8360) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8514..9102) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8594..8613) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9273..10133) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9896..9915 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10134..10238) /gene="bla" /label=AmpR promoter primer_bind 10306..10324 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" enhancer 10424..308 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc 61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac 121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata 181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc 241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta 301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac 361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc 421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc 481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc 541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc 601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat 661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc 721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc 781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta 841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc 901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc 961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca 1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct 1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc 1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga 1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt 1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg 1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga 1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa 1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct 1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct 1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg 1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 2221 cggacctgca ggaattgatc cgcggccgcg acaccatggc acaattggat tacaaggatg 2281 acgatgacaa gaccggtatg aaccaccagc agcagcagca gcagcagaaa gcgggcgagc 2341 agcagttgag cgagcccgag gacatggaga tggaagcggg agatacagat gacccaccaa 2401 gaattactca gaaccctgtg atcaatggga atgtggccct gagtgatgga cacaacaccg 2461 cggaggagga catggaggat gacaccagtt ggcgctccga ggcaaccttt cagttcactg 2521 tggagcgctt cagcagactg agtgagtcgg tccttagccc tccgtgtttt gtgcgaaatc 2581 tgccatggaa gattatggtg atgccacgct tttatccaga cagaccacac caaaaaagcg 2641 taggattctt tctccagtgc aatgctgaat ctgattccac gtcatggtct tgccatgcac 2701 aagcagtgct gaagataata aattacagag atgatgaaaa gtcgttcagt cgtcgtatta 2761 gtcatttgtt cttccataaa gaaaatgatt ggggattttc caattttatg gcctggagtg 2821 aagtgaccga tcctgagaaa ggatttatag atgatgacaa agttaccttt gaagtctttg 2881 tacaggcgga tgctccccat ggagttgcgt gggattcaaa gaagcacaca ggctacgtcg 2941 gcttaaagaa tcagggagcg acttgttaca tgaacagcct gctacagacg ttatttttca 3001 cgaatcagct acgaaaggct gtgtacatga tgccaaccga gggggatgat tcgtctaaaa 3061 gcgtcccttt agcattacaa agagtgttct atgaattaca gcatagtgat aaacctgtag 3121 gaacaaaaaa gttaacaaag tcatttgggt gggaaacttt agatagcttc atgcaacatg 3181 atgttcagga gctttgtcga gtgttgctcg ataatgtgga aaataagatg aaaggcacct 3241 gtgtagaggg caccataccc aaattattcc gcggcaaaat ggtgtcctat atccagtgta 3301 aagaagtaga ctatcggtct gatagaagag aagattatta tgatatccag ctaagtatca 3361 aaggaaagaa aaatatattt gaatcatttg tggattatgt ggcagtagaa cagctcgatg 3421 gggacaataa atacgacgct ggggaacatg gcttacagga agcagagaaa ggtgtgaaat 3481 tcctaacatt gccaccagtg ttacatctac aactgatgag atttatgtat gaccctcaga 3541 cggaccaaaa tatcaagatc aatgataggt ttgaattccc agagcagtta ccacttgatg 3601 aatttttgca aaaaacagat cctaaggacc ctgcaaatta tattcttcat gcagtcctgg 3661 ttcatagtgg agataatcat ggtggacatt atgtggttta tctaaacccc aaaggggatg 3721 gcaaatggtg taaatttgat gacgacgtgg tgtcaaggtg tactaaagag gaagcaattg 3781 agcacaatta tgggggtcac gatgacgacc tgtctgttcg acactgcact aatgcttaca 3841 tgttagtcta catcagggaa tcaaaactga gtgaagtttt acaggcggtc accgaccatg 3901 atattcctca gcagttggtg gagcgattac aagaagagaa aaggatcgag gctcagaagc 3961 ggaaggagcg gcaggaagcc catctctata tgcaagtgca gatagtcgca gaggaccagt 4021 tttgtggcca ccaagggaat gacatgtacg atgaagaaaa agtgaaatac actgtgttta 4081 aagtattgaa gaactcctcg cttgctgagt ttgttcagag cctctctcag accatgggat 4141 ttccacaaga tcaaattcga ttgtggccca tgcaagcaag gagtaatgga acaaaacgac 4201 cagcaatgtt agataatgaa gccgacggca ataaaacaat gattgagctc agtgataatg 4261 aaaacccttg gacaatattc ctggaaacag ttgatcccga gctggctgct agtggagcga 4321 ccttacccaa gtttgataaa gatcatgatg taatgttatt tttgaagatg tatgatccca 4381 aaacgcggag cttgaattac tgtgggcata tctacacacc aatatcctgt aaaatacgtg 4441 acttgctccc agttatgtgt gacagagcag gatttattca agatactagc cttatcctct 4501 atgaggaagt taaaccgaat ttaacagaga gaattcagga ctatgacgtg tctcttgata 4561 aagcccttga tgaactaatg gatggtgaca tcatagtatt tcagaaggat gaccctgaaa 4621 atgataacag tgaattaccc accgcaaagg agtatttccg agatctctac caccgcgttg 4681 atgtcatttt ctgtgataaa acaatcccta atgatcctgg atttgtggtt acgttatcaa 4741 atagaatgaa ttattttcag gttgcaaaga cagttgcaca gaggctcaac acagatccaa 4801 tgttgctgca gtttttcaag tctcaaggtt atagggatgg cccaggtaat cctcttagac 4861 ataattatga aggtacttta agagatcttc tacagttctt caagcctaga caacctaaga 4921 aactttacta tcagcagctt aagatgaaaa tcacagactt tgagaacagg cgaagtttta 4981 aatgtatatg gttaaacagc caatttaggg aagaggaaat aacactatat ccagacaagc 5041 atgggtgtgt ccgggacctg ttagaagaat gtaaaaaggc cgtggagctt ggggagaaag 5101 catcagggaa acttaggctg ctagaaattg taagctacaa aatcattggt gttcatcaag 5161 aagatgaact attagaatgt ttatctcctg caacgagccg gacgtttcga atagaggaaa 5221 tccctttgga ccaggtggac atagacaaag agaatgagat gcttgtcaca gtggcgcatt 5281 tccacaaaga ggtcttcgga acgttcggaa tcccgttttt gctgaggata caccagggcg 5341 agcattttcg agaagtgatg aagcgaatcc agagcctgct ggacatccag gagaaggagt 5401 ttgagaagtt taaatttgca attgtaatga tgggccgaca ccagtacata aatgaagacg 5461 agtatgaagt aaatttgaaa gactttgagc cacagcccgg taatatgtct catcctcggc 5521 cttggctagg gctcgaccac ttcaacaaag ccccaaagag gagtcgctac acttaccttg 5581 aaaaggccat taaaatccat aactgaggat ccggaattcc gcccccctcc ctcccccccc 5641 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta 5701 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc 5761 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat 5821 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc 5881 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt 5941 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt 6001 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag 6061 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt 6121 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga 6181 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca 6241 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg 6301 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc 6361 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt 6421 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg 6481 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg 6541 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc 6601 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc 6661 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc 6721 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc 6781 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg 6841 acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga agccacccgg 6901 ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagtc gagggctgca 6961 gcgctgcaga ggccgagtgc agaactgctc caaagggacc tcaaggcttt ccgagggaca 7021 ctaggctgac tccatcgagc cagtgtagag ataagcttat cgattagtcc aatttgttaa 7081 agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc 7141 tatagagtac gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg 7201 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc 7261 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac 7321 agggtcgacc ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc 7381 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc 7441 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga 7501 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg 7561 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct 7621 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac 7681 cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 7741 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 7801 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag 7861 cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 7921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 7981 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 8041 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 8101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 8161 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 8221 aaagcttact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 8281 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 8341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 8401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 8461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 8521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 8581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 9001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 9061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 9121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 9181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 9241 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 9301 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 9361 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 9421 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 9481 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 9541 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 9601 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 9661 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 9721 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 9781 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 9841 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 9901 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9961 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 10021 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 10081 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 10141 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 10201 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 10261 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 10321 aggccctttc gtcttcaaga attagcttgg ccattgcata cgttgtatcc atatcataat 10381 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact 10441 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagt //
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